Search results for "density [charge]"

Muon capture revisited

1990

Abstract The problem of inclusive muon capture in nuclei is studied by calculating the capture rate in asymmetric infinite nuclear matter and using the local density approximation to evaluate the capture rates in nuclei. It is shown that the method is rather reliable and allows one to improve on approximations used in the past. The need for a strong nuclear renormalization is shown, reducing the capture rates by about a factor two in medium and heavy nuclei. By using standard effective interactions in the spin-isospin channel one can account for this renormalization and one finds a remarkable overall agreement with the measured capture rates for a large list of nuclei through the periodic t…

Ultrastructure, fractionation and biochemical analysis of Cryptosporidium parvum sporozoites.

1999

Abstract Sporozoites of the apicomplexan parasite Cryptosporidium parvum were subjected to cell disruption and subcellular fractionation using a sucrose density step gradient. With this procedure, highly enriched preparations of the parasite membrane, the micronemes, dense granules and amylopectin granules were produced. No separate fraction containing rhoptries was obtained, however this organelle was found in defined fractions of the gradient, still associated with the apical tip of the sporozoites. Using negative staining, the internal structure of the micronemes was revealed by transmission electron microscopy. Micronemes and dense granules showed characteristic protein compositions by …

The role of cell interactions in the control of RNA synthesis.

1967

Spontaneous cytotoxic activity of eosinophilic granule cells separated from the normal peritoneal cavity ofDicentrarchus labrax

2000

Abstract In this study the spontaneous in vitro cytotoxic activity to tumour cell lines, (K562), by unstimulated sea bass ( Dicentrarchus labrax ) leukocytes was examined by trypan blue exclusion test and lactate dehydrogenase release assay. A high anti-tumour cell line activity of resident peritoneal leukocytes was found at an effector to target ratio (E:T) of 25:1 after incubation for 2 h at 18° C. Rabbit and sheep erythrocytes were not lysed. A low activity was displayed by head kidney and spleen cell populations whereas blood leukocytes revealed no significant activity. The effect of E:T ratio on cytotoxicity as well as microscopy observations suggested that the cytotoxic reaction requi…

Quantification of bacterial subgroups in soil : comparison of DNA extracted directly from soil or from cells previously released by density gradient …

2001

All molecular analyses of soil bacterial diversity are based on the extraction of a representative fraction of cellular DNA. Methods of DNA extraction for this purpose are divided into two categories: those in which cells are lysed within the soil (direct extraction) and those in which cells are first removed from soil (cell extraction) and then lysed. The purpose of this study was to compare a method of direct extraction with a method in which cells were first separated from the soil matrix by Nycodenz gradient centrifugation in order to evaluate the effect of these different approaches on the analysis of the spectrum of diversity in a microbial community. We used a method based on polymer…

Integrating LSTMs with Online Density Estimation for the Probabilistic Forecast of Energy Consumption

2019

In machine learning applications in the energy sector, it is often necessary to have both highly accurate predictions and information about the probabilities of certain scenarios to occur. We address this challenge by integrating and combining long short-term memory networks (LSTMs) and online density estimation into a real-time data streaming architecture of an energy trader. The online density estimation is done in the MiDEO framework, which estimates joint densities of data streams based on ensembles of chains of Hoeffding trees. One attractive feature of the solution is that queries can be sent to the here-called forecast-based point density estimators (FPDE) to derive information from …

Forest of Normalized Trees: Fast and Accurate Density Estimation of Streaming Data

2018

Density estimation of streaming data is a relevant task in numerous domains. In this paper, a novel non-parametric density estimator called FRONT (forest of normalized trees) is introduced. It uses a structure of multiple normalized trees, segments the feature space of the data stream through a periodically updated linear transformation and is able to adapt to ever evolving data streams. FRONT provides accurate density estimation and performs favorably compared to existing online density estimators in terms of the average log score on multiple standard data sets. Its low complexity, linear runtime as well as constant memory usage, makes FRONT by design suitable for large data streams. Final…

Online Density Estimation of Heterogeneous Data Streams in Higher Dimensions

2016

The joint density of a data stream is suitable for performing data mining tasks without having access to the original data. However, the methods proposed so far only target a small to medium number of variables, since their estimates rely on representing all the interdependencies between the variables of the data. High-dimensional data streams, which are becoming more and more frequent due to increasing numbers of interconnected devices, are, therefore, pushing these methods to their limits. To mitigate these limitations, we present an approach that projects the original data stream into a vector space and uses a set of representatives to provide an estimate. Due to the structure of the est…

ADM : A Density And Priority Levels Aware Protocol For Broadcasting In Vehicular Ad-Hoc Networks

2014

The broadcasting communication mode is widely used in Vehicular Ad~hoc Networks (VANETs). It is used for sending emergency messages, road-traffic information or to help routing protocols to determine routes. This communication mode is known to be hard to achieve efficiently since it depends on the network density. Indeed, broadcasting methods may cause network congestion if they are not well designed. This paper introduces a novel Autonomic Dissemination Method (ADM) which delivers messages in accordance with given message classes and network density levels. The proposed approach is based on two steps: an offline optimization process and an online adaptation to the network characteristics. …

Assembly of the Major and the Minor Capsid Protein of Human Papillomavirus Type 33 into Virus-like Particles and Tubular Structures in Insect Cells

1994

Native virions of human papillomaviruses (HPV) can be isolated from genital lesions only in very limited amounts. Recent studies have shown that virus-like particles can be obtained by expression of the capsid proteins using vaccinia virus recombinants or the baculovirus system. We now present the first detailed characterization of virus-like particles of a human papillomavirus associated with malignant genital lesions, HPV-33, produced in high yield using the baculovirus expression system. Assembly of the major capsid protein L1 alone or together with the minor capsid protein L2 has been obtained. Both spherical virus-like particles of 50-60 nm diameter and tubular structures of either 25-…